Expression of Kanamycin resistance introduced byAgrobacteriutn binary vector intoNicotiana tabacum andAtropa belladonna |
| |
Authors: | M Ondrej J Vlasák |
| |
Institution: | (1) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na sádkách 702, 370 05 České Budéjovice, Czechoslovakia |
| |
Abstract: | Kanamycin resistance gene was introduced into tobacco and Atropa belladonna cells by binary vectors, based on Agrobacterium,
by means of inoculation of seedlings. The plasmid pGA472, which carries chimaeric kanamycin resistance gene expressed in plants
was introduced by transformation into A. tumefaciens Bo542, harbouring pTiBo542 plasmid and A. rhizogenes 8196, carrying pRi8196
plasmids and the resulting two strains were used as binary vectors. Tobacco tumors induced by A. tumefaciens Bo542(pGA472)
grew as undifferentiated, kanamycin resistant tissues. Those induced by A. rhizogenes 8196(pGA472) differentiated into transformed
plants. When cultivated in vitro on 200 μg ml-1 kanamycin medium, they showed yellow green sectoring, which was not selected out during vegetative propagation. Atropa belladonna
tissues transformed by both A. tumefaciens Bo542(pGA472) and A. rhizogenes 8196(pGA472) differentiated plants which grew well
on 200 μg ml-1 kanamycin as green, non-sectoring plants; sensitive cells obviously did not divide at all. Selection of Atropa belladonna
transformed tissues on kanamycin medium is much more efficient than selection of transformed tobacco tissues with introduced
kanamycin resistance gene. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|