Sulfur-containing Compounds in Lemna perpusilla 6746 Grown at a Range of Sulfate Concentrations |
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Authors: | Datko A H Mudd S H Giovanelli J Macnicol P K |
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Affiliation: | National Institute of Mental Health, Laboratory of General and Comparative Biochemistry, Bethesda, Maryland 20014. |
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Abstract: | Lemna perpusilla 6746, grown photoautotrophically at a series of sulfate concentrations ranging from 0.32 to 1,000 μm, was labeled to radioisotopic equilibrium with 35SO42−. Sulfur-containing compounds were isolated and purified from the colonies. Radioactivity in each compound was a measure of the amount of that compound present in the tissue. The following compounds were identified and quantitated: inorganic sulfate, glutathione, homocyst(e)ine, cyst(e)ine, methionine, S-methylmethionine sulfonium, S-adenosylmethionine, S-adenosylhomocysteine, cystathionine, chloroformsoluble (presumed to be sulfolipid), protein cyst(e)ine, and protein methionine. γ-Glutamylcyst(e)ine, erythro- and threo-thiothreonine, and S-methylcysteine were not detected. No volatile 35S compounds were formed during plant growth at 1,000 μm sulfate, nor were significant amounts of 35S compounds excreted into the medium. |
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