The production of prostanoids by cultured bovine articular chondrocytes |
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Authors: | D Mitrovic E McCall F Dray |
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Institution: | 1. U18 Inserm, Hopital Lariboisière, 6 rue Guy Patin 75010 Paris, and U.R.I.A., Institut Pasteur, 28 rue de Docteur Roux, 75015 Paris, France |
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Abstract: | Bovine articular chondrocytes, cultured as cell suspensions and monolayers, produced prostaglandin (PG) E2 and PGI2 (assayed as 6 keto PGF1α), rather less PGF2α and irregular quantities of thromboxane (Tx) B2. Addition of foetal calf serum to the medium greatly stimulated PG production (a sixfold increase in PGE2 and a twofold increase in 6 keto PGF1α).Prostanoid production by cell suspension grown in serum-free medium generally plateaued after 24 hours. In the presence of 20% foetal calf serum, prostanoid production in long-term monolayer cultures increased during the first 6 days of culture. Levels of PGE2α levels remained high. Indomethacin (10-6M) inhibited chondrocyte PG production both in the presence and absence of added arachidonic acid (10-4M). Prostanoids produced by chondrocytes may play a role in the modulation of cartilage metabolism . |
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