Luteinizing hormone stimulates the production of prostacyclin by isolated ovarian cell <span class="math"><math><mtext>in</mtext></math></span><span class="math"><math><mtext>vitro</mtext></math></span>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Luteinizing hormone stimulates the production of prostacyclin by isolated ovarian cell $in$ $vitro$

Authors:	Johannes D. Veldhuis Patricia Klase Laurence M. Demers

Affiliation:	1. University of Virginia School of Medicine Departments of Internal Medicine and Obstetrics & Gynecology Division of Endocrinology and Reproductive Biology Charlottesville, Virginia 22908, USA;2. Department of Pathology Pennsylvania State University School of Medicine The Milton S. Hershey Medical Center Hershey, Pennsylvania 17033, USA

Abstract:	Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF₁α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF₁α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE₂ were devoid of effect. The time-dependent in vitro production of 6-keto-PGF₁α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.

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