Parentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers |
| |
Authors: | Agapito J Rodríguez J Herrera-Velit P Timoteo O Rojas P Boettcher P J García F Espinoza J R |
| |
Affiliation: | Instituto Peruano de Energía Nuclear (IPEN), Lima, Perú;. Molecular Biotechnology Unit, Laboratories for Research &Development (LID), Universidad Peruana Cayetano Heredia, Lima, Perú;. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency (IAEA), Vienna, Austria;. Universidad Estadual Paulista de Araçatuba, Sao Paulo, Brazil |
| |
Abstract: | The aim of this study was to assess and apply a microsatellite multiplex system for parentage determination in alpacas. An approach for parentage testing based on 10 microsatellites was evaluated in a population of 329 unrelated alpacas from different geographical zones in Perú. All microsatellite markers, which amplified in two multiplex reactions, were highly polymorphic with a mean of 14.5 alleles per locus (six to 28 alleles per locus) and an average expected heterozygosity ( H E) of 0.8185 (range of 0.698–0.946). The total parentage exclusion probability was 0.999456 for excluding a candidate parent from parentage of an arbitrary offspring, given only the genotype of the offspring, and 0.999991 for excluding a candidate parent from parentage of an arbitrary offspring, given the genotype of the offspring and the other parent. In a case test of parentage assignment, the microsatellite panel assigned 38 (from 45 cases) offspring parentage to 10 sires with LOD scores ranging from 2.19 × 10+13 to 1.34 × 10+15 and Δ values ranging from 2.80 × 10+12 to 1.34 × 10+15 with an estimated pedigree error rate of 15.5%. The performance of this multiplex panel of markers suggests that it will be useful in parentage testing of alpacas. |
| |
Keywords: | alpaca genotyping microsatellites multiplex parentage |
本文献已被 PubMed 等数据库收录! |
|