拟南芥中缺铁反应性microRNAs的鉴定

microRNA是一种非编码蛋白质的小分子RNA,参与了植物生长发育及环境胁迫响应的调控,主要通过对靶基因的负调控去影响生物学过程.基于前人对拟南芥全基因组microRNAs及其靶基因的预测,我们找到了靶向15个缺铁响应基因的22个microRNAs(miR158a、miR164c、miR172a、miR1887、miR2111ab、miR3933、miR395ade、miR414、miR828、miR831、miR837-3P、miR837-5P、miR854abcd、miR857、miR861-5P、miR864-5P).对这些microRNAs的启动子进行分析,发现分别有17、10和4个microRNAs启动子中包含缺铁响应元件IDE1、生长素响应元件和乙烯响应元件.进一步通过Poly(T)adaptor RT-PCR方法对这22个microRNAs在缺铁条件下的表达变化做了检测,结果显示,除miR158a和miR837-5P外的20个microRNAs在缺铁条件下的表达变化都有显著差异,且具有时间依赖性.这20个microRNAs可作为缺铁响应的候选microRNAs.

Identification of microRNAs Responsed to Iron Deficiency in Arabidopsis

microRNA (miRNA) is a non protein coding small molecule RNA (small RNA), involved in plant growth and development or stress response, mainly through negative regulation of target genes to influence biological processes. Based on previous genome wide prediction about microRNAs and their targets in Arabidopsis, we found 22 microRNAs (miR158a, miR164c, miR172a, miR1887, miR2111ab, miR3933, miR395ade, miR414, miR828, miR831, miR837 3P, miR837 5P, miR854abcd, miR857, miR861-5P, miR864-5P) targeting 15 genes related to iron deficiency. Promoter analysis of these microRNAs
showed that there were 17, 10 and 4 microRNAs existing iron deficient responsive element 1 (IDE1), auxin responsive element and ethylene responsive element, respectively. Further, Poly(T) adaptor RT-PCR was used to survey the expression of these
22 microRNAs under iron deficiency. Results showed that 20 microRNAs (except miR158a and miR837 5P) were up regulated significantly depending on time course. In summary, these 20 microRNAs can be considered as candidate microRNAs in response to iron deficiency.