Substrate spectrum of tyrocidine thioesterase probed with randomized peptide N-acetylcysteamine thioesters |
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| Authors: | Xie Guiyang Uttamchandani Mahesh Chen Grace Y J Bu Xianzhang Lin San San Wong Ka Man Yan Weili Yao Shao Q Guo Zhihong |
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| Institution: | Department of Chemistry and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Kowloon, SAR, Hong Kong, China. |
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| Abstract: | Apparent kinetic constants k(cat) and K(m) were determined for tyrocidine thioesterase (TycC TE) using randomized peptide N-acetylcysteamine thioesters as substrate analogues. The enzyme has been found to be adequately active for the synthesis of positional-scanning libraries for novel antibiotic screening with reduced k(cat)/K(m) in the range of 2 to 82 folds lower than that of the wild-type sequence |
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