丝裂霉素C对Erwinia herbicola表达及运转冰核活性蛋白方式的影响

利用添加丝裂霉素C(MMC)于不同培养基中，并在不同培养条件下对草生欧文氏菌10025A(E．herbicola 10025A)诱导培养，首次证实该菌表达冰核活性蛋白的活性及运转分泌冰核活性蛋白方式是不同。研究结果显示，MMC的加入可以提高细菌培养物的冰核活性，该现象可以解释为MMC对E．herbicola的作用能够激活细胞SOS应急系统的反应，诱导细胞合成部分参与修复DNA损伤的酶和蛋白因子，达到对E．herbicola诱导表达冰核蛋白通过高尔基体形成小液泡，向膜外分泌的方式，同时对E．herbicola细胞分裂后的形态也发生明显的影响。这对研究细胞在恶劣环境的生存机制以及获取该条件下的蛋白具有重要的意义，对低温生物的研究也将产生积极地影响。

Effects of Mitomycin C on the Expression and Transport of Ice-nuclei Proteins of Erwinia herbicola

In this paper,Mitomycin C (MMC) was added to different kinds of medium to study the effects of different cultural conditions on the Erwinia herbicola 10025A.For the first time it was confirmed that the expressed activity of the ice-nuclei active protein was different from its transportable manner from the ice nucleation active bacteria (Erwinia herbicola 10025A).The findings indicated that MMC could stimulate the SOS response,and induce the synthesis of some enzymes and proteins,which take part in repairing the damaged DNA.The effects of the MMC on the E.herbicola under different media were different.It could increase the ice nucleation activity of the E.herbicola,forming new small vesicles,which are secreted to the outside of membrane.The importance of this research for study the living mechanism of cells ander poor condition was discussed.