Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-alpha release capability of macrophage cell line RAW 264.7 |
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| Authors: | Shu Chin-Hang Wen Bor-Jiun Lin Ko-Jung |
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| Affiliation: | Department of Chemical and Materials Engineering, National Central University, Chung-Li, Taoyuan, Taiwan 32054. chinshu@cc.ncu.edu.tw |
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| Abstract: | A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-alpha releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture. |
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