Physicochemical and Preclinical Evaluation of a Novel Buccal Measles Vaccine |
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| Authors: | Rikhav P. Gala Carmen Popescu Gregory T. Knipp Robyn R. McCain Ruhi V. Ubale Richard Addo Tuhin Bhowmik Christopher D. Kulczar Martin J. D’Souza |
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| Affiliation: | 1.Vaccine Nanotechnology Laboratory, Department of Pharmaceutical Sciences,Mercer University,Atlanta,USA;2.Roquette America Inc,Geneva,USA;3.Department of Industrial and Physical Pharmacy, College of Pharmacy,Purdue University,West Lafayette,USA;4.Purdue Translational Pharmacology of Bindley Biosciences,Purdue University,West Lafayette,USA;5.Department of Pharmaceutical Sciences,Union University School of Pharmacy,Jackson,USA;6.Takeda Vaccine Business Unit,Bozeman,USA |
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| Abstract: | The aim of this study is to develop an orally disintegrating film (ODF) containing a microparticulate measles vaccine formulation for buccal delivery. The measles vaccine microparticles were made with biocompatible and biodegradable bovine serum albumin (BSA) and processed by spray drying. These vaccine microparticles were incorporated in the ODF, consisting of Lycoat RS720®, Neosorb P60W® and Tween 80. The yield of the microparticles was approximately 85–95%, w/w. The mean size of the vaccine microparticles was 3.65?±?1.89 μm and had a slightly negative surface charge of 32.65?±?2.4 mV. The vaccine particles were nontoxic to normal cells at high concentrations (500 μg/2.5?×?105 cells) of vaccine particles. There was a significant induction of innate immune response by vaccine microparticles which was observed in vitro when compared to blank microparticles (P?
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