Adaptation of the yeast Yarrowia lipolytica to ethanol |
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Authors: | E. N. Biryukova A. Yu. Arinbasarova A. G. Medentsev |
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Affiliation: | (1) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia |
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Abstract: | Resistance of the yeast Yarrowia lipolytica to ethanol stress was studied under different ethanol concentrations and treatment duration. Cell viability was shown to increase in the variants including preliminary treatment with small doses of ethanol, oxidants, or soft thermal exposure. The study of the respiratory activity under ethanol stress revealed the involvement of an alternative cyanide-resistant oxidase in the adaptive response of the cells. The level of intracellular cAMP decreased in response to the action of ethanol, which correlated with increased activity of the antioxidant systems (catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, glutathione reductase) and NAD+-dependent alcohol dehydrogenase. |
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Keywords: | Yarrowia lipolytica stress adaptation ethanol respiratory activity inhibitor analysis alternative oxidase cAMP NAD+-dependent alcohol dehydrogenase |
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