Application of a membrane recycle bioreactor for continuous ethanol production |
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Authors: | Karel Melzoch Mojmír Rychtera Nikolay S. Markvichov Věra Pospíchalová Gabriela Basařová Michail N. Manakov |
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Affiliation: | (1) Department of Fermentation Chemistry and Bioengineering, Prague Institute of Chemical Technology, 166 28 Prague, Czechoslovakia;(2) Department of Industrial Biotechnology, D.I. Mendeleev Moscow Institute of Chemical Technology, Moscow, USSR |
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Abstract: | Summary A series of continuous fermentations were carried out with a production strain of the yeast Saccharomyces cerevisiae in a membrane bioreactor. A membrane separation module composed of ultrafiltration tubular membranes retained all biomass in a fermentation zone of the bioreactor and allowed continuous removal of fermentation products into a cell-free permeate. In a system with total (100%) cell recycle the impact of fermentation conditions [dilution rate (0.03–0.3 h–1); substrate concentration in the feed (50–300 g·1–1); biomass concentration (depending on the experimental conditions)] was studied on the behaviour of the immobilized cell population and on ethanol formation. Maximum ethanol productivity (15 g·1–1·h–1) was attained at an ethanol concentration of 81 g·1–1. The highest demands of cells for maintenance energy were found at the maximum feed substrate concentration (300 g·1–1) and at very low concentrations of cells in the broth. |
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