Identification of genomic differences between Campylobacter jejuni subsp. jejuni and C. jejuni subsp. doylei at the nap locus leads to the development of a C. jejuni subspeciation multiplex PCR method |
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| Authors: | William G Miller Craig T Parker Sekou Heath and Albert J Lastovica |
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| Institution: | (1) Produce Safety and Microbiology Research Unit, Agricultural Research Service U.S. Department of Agriculture, , 94710 Albany, CA, USA;(2) Department of Biotechnology, University of the Western Cape, Bellville, South Africa |
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| Abstract: | Background The human bacterial pathogen Campylobacter jejuni contains two subspecies: C. jejuni subsp. jejuni (Cjj) and C. jejuni subsp. doylei (Cjd). Although Cjd strains are isolated infrequently in many parts of the world, they are obtained primarily from human clinical samples and
result in an unusual clinical symptomatology in that, in addition to gastroenteritis, they are associated often with bacteremia.
In this study, we describe a novel multiplex PCR method, based on the nitrate reductase (nap) locus, that can be used to unambiguously subspeciate C. jejuni isolates. |
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