Vero细胞微载体不同培养方法的评价

对三种不同培养方法进行细胞生长速度、密度、营养及代谢产物浓度的比较分析，以优化和筛选最佳培养条件与方式。用同体积生物反应罐，基本培养条件相同，采用批培养、再循环培养、灌流培养三种方式进行了Vero细胞微载体（CytodexI）的周期培养。三种培养方法均达到预期效果，最终细胞密度分别为每毫升2.09×10

Evaluation of different Vero cells on microcarriers culture systems

Vero cells were cultivated periodically on microcarriers Cytodex I by using batch culture mode, recirculation culture mode and perfusion culture mode, respectively in the bioreactor of the same volume 7L and under the basic cultivation conditions. Expected result were obtained for three culture modes. Their final cell densities were: 2.09×106 cells/ml; 3.37×106 cells/ml; 4.34×106cells/ml; while the perfusion cultures doubling level was the highest of 5.59; the doubling time was the least of 30.05 hour. The experimental data indicated that the perfusion culture, was excelled than that of the recirculation culture, and that the recirculation culture which had the characteristics of both perfusion and batch cultures was excelled than that of the batch culture.