Synthetic biology tools for engineering Yarrowia lipolytica |
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Authors: | M. Larroude T. Rossignol J.-M. Nicaud R. Ledesma-Amaro |
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Affiliation: | 1. Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France;2. Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, United Kingdom |
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Abstract: | The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools. |
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Keywords: | Synthetic biology DNA assembly CRISPR-Cas9 Genome editing Golden Gate Industrial biotechnology Genome scale metabolic models Metabolic engineering |
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