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Discovering novel hydrolases from hot environments
Authors:Roland Wohlgemuth  Jennifer Littlechild  Daniela Monti  Kirk Schnorr  Teunke van Rossum  Bettina Siebers  Peter Menzel  Ilya V. Kublanov  Anne Gunn Rike  Georgios Skretas  Zalan Szabo  Xu Peng  Mark J. Young
Affiliation:1. Sigma-Aldrich, Member of Merck Group, Industriestrasse 25, CH-9470 Buchs, Switzerland;2. Biocatalysis Centre, University of Exeter, The Henry Wellcome Building for Biocatalysis, Stocker Road, Exeter EX4 4QD, United Kingdom;3. Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131 Milano, Italy;4. Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark;5. Wageningen University and Research, Laboratory of Microbiology, Stippeneng 4, 6708 WE Wageningen, the Netherlands;6. Molecular Enzyme Technology and Biochemistry, Biofilm Centre, Faculty of Chemistry, University of Duisburg-Essen, Universitaetsstr. 5, S05 V03 F41, 45141 Essen, Germany;7. Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 København, Denmark;8. Extremophiles Metabolism Laboratory, Winogradsky Institute of Microbiology, Research Center of Biotechnology, RAS, Prospekt 60 Letiya Oktyabrya 7, Moscow, Russia;9. Norwegian Geotechnical Institute, Sognsveien 72, N-0855 Oslo, Norway;10. Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, 48 Vassileos Constantinou Ave, 11635 Athens, Greece;11. MicroDish BV, Padualaan 8, 3584 CH Utrecht, the Netherlands;12. Thermal Biology Institute, Departments of Microbiology and Plant Sciences, 111A Chemistry, Montana State University, Bozeman, MT 59717, USA;13. Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, 13125 Berlin, Germany;14. U-Protein Express BV, Yalelaan 62, 3584 CH Utrecht, the Netherlands;15. Institute of Technical Biochemistry, Lodz University of Technology, ul. Stefanowskiego 4/10, 90-924 Lodz, Poland;p. Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, Netherlands;q. Standard Norge, Mustads vei 1, 0283 Oslo, Norway
Abstract:
Keywords:Hydrolases  Metagenomics  Enrichment  Sequencing  Genomics  Thermophiles  Gene expression  Enzyme screening  Enzyme characterization  Biocatalysis
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