|
|||||
|
|
Review: In vitro generation of red blood cells for transfusion medicine: Progress,prospects and challenges |
|
| Authors: | Esmond Lee Jaichandran Sivalingam Zhong Ri Lim Gloryn Chia Low Gin Shi Mackenna Roberts Yuin-Han Loh Shaul Reuveny Steve Kah-Weng Oh |
| Institution: | 1. Stem Cell Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore 138668, Republic of Singapore;2. Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Palo Alto, CA 94305, USA;3. Brilliant Research Pte. Ltd, Singapore 139955, Republic of Singapore;4. Oxford-University College London Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, UK;5. Institute of Molecular and Cellular Biology, Agency for Science, Technology and Research, Singapore 138668, Republic of Singapore;6. Department of Biological Sciences, National University of Singapore, Singapore 117543, Republic of Singapore |
| Abstract: | In vitro generation of red blood cells (RBCs) has the potential to circumvent the shortfalls in global demand for blood for transfusion applications. The conventional approach for RBC generation has been from differentiation of hematopoietic stem cells (HSCs) derived from cord blood, adult bone marrow or peripheral blood. More recently, RBCs have been generated from human induced pluripotent stem cells (hiPSCs) as well as from immortalized adult erythroid progenitors. In this review, we highlight the recent advances to RBC generation from these different approaches and discuss the challenges and new strategies that can potentially make large-scale in vitro generation of RBCs a feasible approach. |
| Keywords: | Red blood cells Hematopoietic Induced pluripotent stem cells Immortalized Erythroid Cord blood 2 3 DPG 2 3 diphosphoglcerate AIDS Acquired immune deficiency syndrome ATP adenosine triphosphate BM bone marrow cRBCs cultured red blood cells CB cord blood CFSE carboxyfluorescein succinimidyl ester EB embryoid body FDA US Food and Drug Administration G6PDH Glucose 6-phosphate dehydrogenase G-CSF granulocyte colony-stimulating factor GMP good manufacturing practise HBOC hemoglobin-based oxygen carriers HbF fetal hemoglobin HbA adult hemoglobin HSCs hematopoietic stem cells hESCs human embryonic stem cells hiPSCs human induced pluripotent stem cells hPSCs human pluripotent stem cells HSPCs hematopoietic stem and progenitor cells HiDEP human iPS cell-derived erythroid progenitor lines HUDEP human umbilical cord blood-derived erythroid progenitor lines iEP induced erythroid progenitors imERYPC immortalized erythrocyte progenitor cell ImEry immortalized erythroblast cell line MCV mean corpuscular volume MCH mean corpuscular hemoglobin MCHC mean corpuscular hemoglobin concentration MSCs mesenchymal stem cells PB peripheral blood PK Pyruvate kinase RBCs red blood cells |
| 本文献已被 ScienceDirect 等数据库收录！ | |