| 基于多重PCR的DNA Marker制备方法 |
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| 引用本文： | 张俊河,王俐,董卫华,王芳,王天云. 基于多重PCR的DNA Marker制备方法[J]. 生物技术通报, 2010, 0(12) |
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| 作者姓名： | 张俊河 王俐 董卫华 王芳 王天云 |
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| 作者单位： | 新乡医学院生物化学与分子生物学教研室,新乡,453003 |
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| 基金项目： | 河南省科技厅科技攻关资助项目(0624410041); 河南省教育厅自然科学基金(2008A310008) |
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| 摘 要： | 报道了一种简便的制备分子量大小为100-1000bp DNA marker的方法,其原理是以一段特异的DNA片段为模板,设计PCR引物,采用多重PCR的方法一次扩增100-1000bp系列条带,酚/氯仿抽提,乙醇沉淀,即可得到条带清晰的DNA marker。
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| 关 键 词： | DNA marker PCR 凝胶电泳 制备 |
A Simple Method for Prepareing DNA Marker |
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| Zhang Junhe,Wang Li,Dong Weihua,Wang Fang,Wang Tianyun. A Simple Method for Prepareing DNA Marker[J]. Biotechnology Bulletin, 2010, 0(12) |
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| Authors: | Zhang Junhe Wang Li Dong Weihua Wang Fang Wang Tianyun |
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| Affiliation: | Zhang Junhe Wang Li Dong Weihua Wang Fang Wang Tianyun(Department of Biochemistry and Molecular Biology,Xinxiang Medical University,Xinxiang 453003) |
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| Abstract: | A simple method that prepare DNA marker is reported in this paper.A specific DNA fragment was used as PCR template,and multiple PCR was used to amplify 100-1 000 bp series DNA fragments,and then were purified and electrophoresised in 2% agarose gels,the bands of the prepared DNA marker were clear and could be used in the molecular experiments. |
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| Keywords: | DNA marker PCR Gel electrophoresis Preparation |
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