Conjugative transfer of raffinose metabolism in Lactococcus lactis |
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Authors: | William J Kelly Graham P Davey Lawrence J.H Ward |
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Affiliation: | Horticulture and Food Research Institute, Palmerston North Research Centre, Private Bag 11030, Palmerston North, New Zealand;New Zealand Dairy Research Institute, Palmerston North, New Zealand |
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Abstract: | Lactococcus lactis subsp. lactis strains isolated from various sprouted seed products were able to transfer the ability to ferment raffinose in conjugation experiments at frequencies between 10−4 and 10−7 per donor cell. There was no evidence of plasmid transfer, but pulsed-field gel electrophoresis analysis showed that all transconjugants had acquired large chromosomal insertions indicative of conjugative transposons. Raffinose transconjugants contained inserts of 45 or 60 kb at one of two chromosomal sites, and these inserts contained two copies of an element related to the lactococcal insertion sequence ISS1. |
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Keywords: | Lactococcus lactis Raffinose metabolism Pulsed-field gel electrophoresis ISS1 Conjugative transposons |
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