DA rats from two colonies differ genetically and in their arthritis susceptibility |
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Authors: | Carola Rintisch Rikard Holmdahl |
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Institution: | (1) Medical Inflammation Research, Biomedical Center, Lund University, 22184 Lund, Sweden;(2) Medical Inflammation Research, Department of Medical Biochemistry and Biophysics, Karolinska Institute, 17177 Stockholm, Sweden |
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Abstract: | The arthritis-susceptible DA rat is one of the most commonly used rat strains for genetic linkage analysis and is instrumental
for the identification of many genetic loci. Even though DA rats were kept as inbred lines at different institutes and suppliers,
it became obvious that the various breeding stocks differed genetically. To be able to compare the results from different
linkage studies it is very import to verify the genetic background of the substrains used in those studies. We performed a
genetic and phenotypic analysis of two DA substrains, DA/ZtmRhd and DA/OlaHsd, and found several genetic differences. One
of the allelic differences between the DA/ZtmRhd and the DA/OlaHsd strain was located at rat chromosome 3, a 17-Mb large fragment,
including the peak marker of a previously identified quantitative trait locus (QTL) for collagen-induced arthritis, Cia11. In addition, the substrains exhibited a significant difference in the susceptibility to pristane-induced arthritis (PIA)
and disease severity of collagen-induced arthritis and PIA. However, by generating and testing a congenic line, we could demonstrate
that phenotypic differences were not due to the contaminating fragment on chromosome 3. Nevertheless, we conclude that DA
substrains show distinct genetic differences and caution should be taken when comparing arthritis data from different DA substrains.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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