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Preservation of Neisseria genus microbes by a deep freezing method]
Authors:N P Zaliubovskaia  R I Kiselev
Abstract:A method of meningococcus and of vaccine gonococcus strains preservation under conditions of deep freezing with polyethyleneoxide of mol mass 400 was developed. Peculiarities of phasic transitions were studied by the method of differential thermal analysis; hydration properties of polyethyleneoxide were investigated by nuclear magneticresonance (spin-echo). The main cultural and biochemical properties after freezing and after one year of storage in 8 strains of the microbes under study remained unchanged; their survival constituted not less than 90%.
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