PKA controls a level of topoisomerase I mRNA in mouse L5178Y lymphoma cells treated with db-cAMP |
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| Authors: | R. M. Czerwinski K. Nieznanski B. Sochanowicz K. Staron |
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| Affiliation: | (1) Institute of Biochemistry, Warsaw University, Warsaw, Poland;(2) Institute of Nuclear Chemistry and Technology, Warsaw, Poland |
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| Abstract: | The level of topoisomerase I mRNA was measured in cells of two mouse lymphoma (LY) sublines treated with db-cAMP. A transient increase of the level was observed to be of about 60% of the basic level and to have maximum after the 3 h treatment of LY-S cells. The increase in LY-R subline was two-fold lower. The activity of PKA in a cytosol fraction of LY-S cells was 1.75 times higher than that in LY-R cells. The activity of PKA in membranes and nuclear fraction did not differ significantly in both cell types. When the activity of PKA in LY-S cells was inhibited with H8, no increase of the level of topoisomerase I mRNA was observed upon db-cAMP treatment of cells. We suggest that the activity of PKA in the cytosol controls the expression of topoisomerase I gene in LY cells at high concentration of cAMP.Abbreviations db-cAMP dibutyryl-cAMP - H8 N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide - LY mouse L5178Y lymphoma - PKA protein kinase A - topo I topoisomerase I |
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| Keywords: | topoisomerase 1 mRNA db-cAMP PKA activity mouse L5178Y lymphoma sublines |
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