Chromoplasts of Tropaeolum majus L.: Lipid synthesis in whole organelles and subfractions |
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Authors: | B Liedvogel H Kleinig J A Thompson H Falk |
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Institution: | (1) Lehrstuhl für Zellbiologie, Institut für Biologie II, Schänzlestraße 1, D-7800 Freiburg, Federal Republic of Germany |
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Abstract: | Isolation of tubulous chromoplasts from Tropaeolum majus L. petals was achieved in pure form. Their main substructures-lipid bodies, tubules, and envelope membranes-have been enriched. Whole chromoplasts as well as substructures have been tested for their activities in lipid synthesis. The following activities were found: fatty acid synthesis from acetate, glycosyl transfer reactions from UDP-galactose and UDP-glucose to galactolipids and sterols, acyltransferase reactions from palmitoyl-CoA, and a very active acyl-CoA hydrolase (EC 3.1.2.2.). Fatty acid synthesis was restricted to whole chromoplasts. Glycosyl- and acyltransferases were essentially confined to envelope membranes, whereas acyl-CoA hydrolase was found in all fractions. The chemical composition of chromoplast subfractions was determined. The lipid bodies consisted mainly of galactolipids and carotenoid esters in a 1:1 ratio, together with small amounts of protein. |
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Keywords: | Acyl-CoA hydrolase Acyltransferase Chromoplasts Galactosyltransferase Tropaeolum |
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