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The first lines of divergence in the Bacteria domain seem to be the hyperthermophilic organisms: a check using an outgroup of sequences from mesophiles in phylogenetic analysis
Authors:Sergio Branciamore  Sacha Barion  Enzo Gallori  Massimo Di Giulio
Institution:1. Department of Animal Biology and Genetics, University of Florence, Via Romana 17/19, 50125, Firenze, Italy
2. Laboratory of Molecular Evolution, Institute of Genetics and Biophysics ‘Adriano Buzzati Traverso’, CNR, Via P. Castellino, 111, 80131, Napoli, Italy
Abstract:We have conducted a check by substituting, in a previous phylogenetic analysis, an outgroup of sequences from hyperthermophilic archaea with another of mesophilic sequences of archaea. This should remove a possible compositional bias which might be responsible for the deep position of Thermotogales and Aquificales in the Bacteria domain, as observed in previous analyses. This check brought to light a weak compositional bias which does not seem, however, to entirely explain the deep position occupied by hyperthermophilic bacteria. The present analysis also seems to show that Planctomycetes are one of the deepest lines of divergence in the Bacteria domain, although they do not seem to be the very deepest.
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