Characterisation and inhibition effect of cetrimide on collagenase produced byAspergillus flavus, isolated from mycotic ulcers |
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| Authors: | Yehia A -G Mahmoud Soad Mohamed Abu El-Souod Sameh Mohamed El-Shourbagy Anwer S M El-Badry |
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| Institution: | 1. Mycology Research Unit, Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
2. Ophthalmology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
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| Abstract: | Collagenase was found to be the most important enzyme, produced by mycotic keratitis fungi. Therefore,Aspergillus flavus collagenase enzyme has been purified by ammonium sulphate precipitation, Sephadex G25 and DEAE-cellulose chromatography. Electrophoretic analysis for the purified enzyme indicated one subunit of molecular weight of 70–90 KDa when examining on SDS-PAGE. Cetrimide (cetyl trimethyl ammonium bromide) has been tested against the purified collagenase enzyme and indicated reversible competitive inhibitor (kis=0.15 mg/ml) with high promising activity. Cetrimide might be used to inhibit mycotic keratitis fungi. |
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