Ultrastructural and protein changes in cell suspension cultures of peach associated with low temperature-induced cold acclimation and abscisic acid treatment |
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Authors: | Rajeev Arora Michael E Wisniewski |
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Institution: | (1) Appalachian Fruit Research Station, United States Department of Agriculture-Agricultural Research Service, 45 Wiltshire Road, 25430 Kearneysville, WV, USA;(2) Present address: Division of Plant & Soil Sciences, West Virginia University, 26506 Morgantown, WV, USA |
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Abstract: | Cell suspension cultures were initiated from callus derived from xylem tissues of peach Prunus persica (L.) Batsch]. Cold acclimation was induced (LT50 of-13°C) in cell suspensions at 3°C in the dark for 10 days. Freezing tolerance returned to the level of nonacclimated cells (LT50 of –4.5°C) when cold-acclimated cells were transferred to 24°C (in dark) for 3 days. Addition of 75 M abscisic acid (ABA) to the growth medium failed to induce cold acclimation after cells were cultured for 5 days at 24°C. Microvacuolation, cytoplasmic augmentation and disappearance of starch grains were observed in cells that were cold-acclimated by exposure to low temperature. Similar ultrastructural alterations were not observed in ABA-treated cells. Several qualitative and quantitative changes in proteins were noted during both cold acclimation and ABA treatment. Both the ultrastructural and protein changes observed during cold acclimation were reversed during deacclimation. The relationship of these changes to cold acclimation in peach cell-cultures is discussed.Abbreviations ABA
abscisic acid
- 2,4-d
2,4-dichlorophenoxyacetic acid
- IBA
indole-3-butyric acid
- Ms
Murashige & Skoog
- PMSF
phenylmethylsulfonyl fluoride
- LT50 or Freezing Tolerance
temperature that resulted in 50% decrease in TTC reduction
- TTC
2,3,5-triphenyltetrazolium chloride |
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Keywords: | freezing tolerance LT50 nonacclimated Prunus persica |
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