Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties. |
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| Authors: | G Amicosante A Oratore N Franceschini M Maccarrone R Strom M Galleni and J M Frère |
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| Institution: | Università degli Studi dell'Aquila, Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Italy. |
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| Abstract: | Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected. |
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