Proteomic analysis of log to stationary growth phase Lactobacillus plantarum cells and a 2-DE database |
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Authors: | Cohen David P A Renes Johan Bouwman Freek G Zoetendal Erwin G Mariman Edwin de Vos Willem M Vaughan Elaine E |
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Affiliation: | Laboratory of Microbiology, Wageningen University and Research Center, Wageningen, The Netherlands. David.Cohen@wur.nl |
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Abstract: | Lactobacillus plantarum is part of the natural microbiota of many food fermentations as well as the human gastro-intestinal tract. The cytosolic fraction of the proteome of L. plantarum WCFS1, whose genome has been sequenced, was studied. 2-DE was used to investigate the proteins from the cytosolic fraction isolated from mid- and late-log, early- and late-stationary phase cells to generate reference maps of different growth conditions offering more knowledge of the metabolic behavior of this bacterium. From this fraction, a total of 200 protein spots were identified by MALDI-MS and a proteome production map was constructed to facilitate further studies such as detection of suitable biomarkers for specific growth conditions. More than half (57%) of the identified proteins were predicted to be involved in metabolic pathways of the bacterium. The protein profile changed during the growth of the bacteria such that 29% of the identified proteins involved in anabolic pathways were at least twofold up-regulated throughout the mid- and late-exponential and early-stationary phases. In the late-stationary phase, six proteins involved in stress or with a potential role for survival during starvation were up-regulated significantly. |
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Keywords: | 2‐DE Differential production Growth phases Lactobacillus plantarum |
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