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An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Incidence and influence on baculovirus activity |
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| Authors: | PV Vail TJ Morris SS Collier B Mackey |
| Institution: | Stored Product Insects Research Laboratory, U.S. Department of Agriculture-ARS, 5578 E. Air Terminal Drive, Fresno, California 93727 USA;Department of Plant Pathology, University of California, 333 Hilgard Hall, Berkeley, California 94710 USA;Stored Product Insects Research Laboratory, U.S. Department of Agriculture-ARS, 5578 E. Air Terminal Drive, Fresno, California 93727 USA;Biometrical and Statistical Services Staff, U.S. Department of Agriculture-ARS, Western Regional Research Center, 800 Buchanan Street, Berkeley, California 94710 USA |
| Abstract: | A small RNA virus infectious to Trichoplusia ni larvae (TRV) was observed as a contaminant of several Autographa californica nuclear polyhedrosis virus preparations (AcMNPV). The extent of contamination in various AcMNPV preparations was studied by means of serial enrichment passages through T. ni larvae and enzyme-linked immunosorbent assay (ELISA). TRV could not be detected by ELISA in the original preparation of AcMNPV polyhedra prepared in 1968 even after five enrichment passages. Antibody inactivation offers a possible prophylactic method against TRV but temperature inactivation (55°C) does not. Although TRV reduced larval weight, it had little or no effect on bioassays of AcMNPV to T. ni and Heliothis virescens. |
| Keywords: | RNA virus nuclear polyhedrosis virus enzyme-linked immunosorbent assay (ELISA) infectivity |
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