Differential staining of insect neurons with nickel and cobalt |
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Authors: | Masaki Sakai Tsuneo Yamaguchi |
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Affiliation: | Department of Biology, Faculty of Science, Okayama University, Tsushima, Okayama 700, Japan |
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Abstract: | Pairs of neurons were differentially stained by intracellular injection of the divalent cations, nickel and cobalt. Pairs of neuronal tracts could be similarly labelled following immersion of their axon bundles in the markers described. Use of nickel and a 4 : 6 mixture of cobalt and nickel according to the protocols presented here revealed the morphology of cricket neuron processes as thin as 1 μm in diameter and yielded a favourable contrast between the cells marked, as well as with respect to the background of unstained tissue. |
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Keywords: | Insect neuron differential marking cobalt nickel backfilling intracellular injection intensification |
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