Determination of heat-stable exotoxin of Bacillus thuringiensis by high-performance anion-exchange chromatography |
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| Authors: | Svetlana N. Bubenschikova Vera K. Kagramanova Ludmila A. Baratova Elvira B. Danilova Elena B. Kruglyak |
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| Affiliation: | M. V. Lomonosov Moscow State University, Moscow, USSR;All-Union Research Institute of Microbiological Methods for Plant Protection and Bacterial Preparations Moscow, USSR |
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| Abstract: | A high-performance anion-exchange chromatography method for quantitative determination of heat-stable exotoxin in insecticide preparations and cultivation liquids is described. Heat-stable exotoxin-containing samples were chromatographed in a column packed with anion-exchange resin under gradient elution conditions, using HCl and NaCl solution, at 50°C. The calibration was linear in the exotoxin concentration range from 1 to 100 mg/ml. The sensitivity of the method allows one to determine 1 nmol and more of the heat-stable exotoxin in the applied sample, the relative standard deviation being not more than ±4%. |
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| Keywords: | Heat-stable exotoxin β-exotoxin high-performance liquid chromatography |
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