Ecdysteroid synthesis by the ring gland of Drosophila melanogaster during late-larval,prepupal and pupal development |
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Authors: | Christopher P.F. Redfern |
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Affiliation: | Department of Molecular Biology, University of Edinburgh, King''s Buildings, Mayfield Road, Edinburgh EH9 3JR, U.K. |
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Abstract: | Radioimmunoassay has been used to determine the characteristics of ecdysteroid synthesis by ring glands and brain-ring gland preparations from late 3rd-instar larvae of Drosophila melanogaster cultured in vitro. The rate of synthesis and secretion is linear for at least 4 hr in culture. Using a 4-hr culture period, variation in the rate of ecdysteroid synthesis by brain-ring gland preparations during larval, prepupal and pupal development has been examined. The rate of synthesis and secretion is highest in late 3rd-instar larvae and decreases after puparium formation. During pupal development, at a time when the endogenous ecdysteroid titre is again increasing, the rate of ecdysteroid synthesis by brain-ring gland preparations remains low and is only 10% of that prior to puparium formation. It is, therefore, likely that the ring gland is not a major source of ecdysteroids during this period. |
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Keywords: | Ecdysteroid secretion ring gland |
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