In vitro production of zoospores by the mosquito pathogen Lagenidium giganteum (Oomycetes: Lagenidiales) on solid media |
| |
Authors: | S. Jaronski R.C. Axtell S.M. Fagan A.J. Domnas |
| |
Affiliation: | Entomology Department, North Carolina State University, Raleigh, North Carolina 27650 USA;Biochemistry Laboratory, Botany Department, University of North Carolina, Chapel Hill, North Carolina 27514 USA |
| |
Abstract: | Reliable, large-scale production of Lagenidium giganteum zoospores was obtained on solid media. The fungus was grown for 7 days in a liquid medium of wheat germ, hemp seed, yeast extract, and glucose, then placed onto hemp-seed agar. Zoosporogenesis was induced on agar by immersing the fungal cultures into water. Zoospore production began 10 hr postimmersion, peaked at 18 hr, and ceased by 36 hr. A single, 10-cm Petri dish of fungus on hemp-seed agar produced 1.7?3.8 × 107 zoospores during the 26 hr of zoosporogenesis. Optimal zoospore production occurred with 4- to 7-day-old cultures; cultures older than 10 days produced few zoospores. The temperature range for zoosporogenesis was 15–35°C. The extent of zoosporogenesis was directly related to the volume of water used to induce zoospore formation and inversely proportional to agar thickness. Bioassay of zoospores against second instar Culex quinquefasciatus larvae yielded an LD50 of 400 zoospores/ml. |
| |
Keywords: | zoospore biological control mosquitoes |
本文献已被 ScienceDirect 等数据库收录! |
|