Development of microsatellite markers from tartary buckwheat |
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Authors: | Yan-Qin Li Tong-Lin Shi Zong-Wen Zhang |
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Affiliation: | (1) Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, P. R. China;(2) IPGRI Office for East Asia, c/o The Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China |
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Abstract: | A genomic library enriched with (gT)n repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5′-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed that the degenerate primer was reliably anchoring at the 5′-end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of tartary buckwheat. |
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Keywords: | Anchored PCR Degenerate primer Fagopyrum tataricum Microsatellite Plants diversity |
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