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Mapping the distribution of 3-hydroxy oxylipins in the ascomycetous yeast Saturnispora saitoi |
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| Authors: | Bareetseng Andries S Kock Johan L F Pohl Carolina H Pretorius Elma E Strauss Catharina J Botes Piet J Van Wyk Pieter W J Nigam Santosh |
| Affiliation: | aDepartment of Microbial, Biochemical and Food Biotechnology, UNESCO MIRCEN: Industrial Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, South Africa;bCentre for Confocal and Electron Microscopy, University of the Free State, P.O. Box 339, Bloemfontein, South Africa;cEicosanoid Research Division, Department of Gynaecology, Berlin, University Medical Centre Benjamin Franklin, Free University, D-12200 Berlin, Germany |
| Abstract: | The distribution of 3-hydroxy oxylipins in Saturnispora saitoi was mapped using immunofluorescence microscopy. Fluorescence was observed on aggregating ascospores, indicating the presence of 3-hydroxy oxylipins on the surface or between ascospores. The oxylipin was identified as 3-hydroxy 9:1 using gas chromatography mass spectrometry. Furthermore, ultrastructural studies using scanning and transmission electron microscopy on ascospores revealed a clear equatorial ledge surrounding oval-shaped ascospores. |
| Keywords: | Ascospores Electron microscopy 3-Hydroxy oxylipins Immunofluorescence Saturnispora saitoi Ultrastructure Yeast |
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