Immunohistochemical evidence for the presence of tryptophan hydroxylase in the brains of insects as revealed by sheep anti-tryptophan hydroxylase polyclonal antibody |
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Authors: | Xuexiang Bao Ximei Tian Zhifu Zhao Yutang Qu Bin Wang Jinbei Zhang Tianyi Liu Lina Yang Jiye Lv Chuantao Song |
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Institution: | (1) Laboratory of Insect Brain Neurobiology, School of Life Sciences, Northeast Normal University, Changchun, 130024, People’s Republic of China;(2) Key Laboratory for Terrain-Machine Bionics Engineering (Ministry of Education, China), Jilin University at Nanling Campus, Changchun, 130022, People’s Republic of China;(3) Jilin Province Sericultural Scientific Research Institute, Jilin, 132200, People’s Republic of China;(4) School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, People’s Republic of China |
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Abstract: | Immediately following the discovery of tryptophan hydroxylase in Drosophila, we demonstrated the presence of tryptophan hydroxylase in the brain of the beetle Harmonia axyridis (Coleoptera: Coccinellidae). However, whether tryptophan hydroxylase is present in the brains of other insects is still a
matter of discussion. In the current study, sheep anti-tryptophan hydroxylase polyclonal antibody has been applied to test
for tryptophan hydroxylase immunoreactivity in a broader taxonomic range of insect brains, including holometabolous and hemimetabolous
insects: one species each of Coleoptera, Hymenoptera, Diptera, and Blattaria, and two species of Lepidoptera. All species
show consistent tryptophan hydroxylase immunoreactivity with distribution patterns matching that of serotonin. The immuno-positive
results of such an antibody in brains from diverse orders of insects suggest that specific tryptophan hydroxylase responsible
for central serotonin synthesis is probably present in the brains of all insects.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
This work was supported by grants from the National Natural Science Foundation of China (grant no. 30470546) and the Natural
Science Foundation of Jilin Province (grant no. 20030550–7). |
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Keywords: | Tryptophan hydroxylase Serotonin Immunohistochemistry Aromatic amino acid hydroxylase Brain Insects |
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