Callus formation from protoplasts of culturedSpinacia oleracea cells |
| |
| Authors: | H Nakagawa H Tanaka T Oba N Ogura M Iizuka |
| |
| Institution: | (1) Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University, 640 Matsudo, Japan;(2) Department of Horticultural Science, Faculty of Horticulture, Chiba University, 640 Matsudo, Japan |
| |
| Abstract: | Cell suspensions were initiated from plumule derived calli ofSpinacia oleracea. Some of these cell lines could be maintained in culture for at least three years without a reduced growth rate. A high yield of protoplasts was obtained from the cell suspensions. When protoplasts were cultured in Murashige and Skoog medium with naphthaleneacetic acid and 6-benzyladenine, cell wall formation was observed after three days. The cultured protoplasts produced numerous cell-clusters within two weeks. However only protoplasts isolated from suspensions which were in a rapidly dividing phase were able to divide with a high frequency and give rise to callus colonies. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
