首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Generation of marker-free transgenic maize by regular two-border <Emphasis Type="Italic">Agrobacterium</Emphasis> transformation vectors
Authors:Shihshieh Huang  Larry Gilbertson  Tom Adams  Kathleen Malloy  Emily Reisenbigler  Darren Birr  Mark Snyder  Qiang Zhang  Michael Luethy
Institution:(1) Mystic Research, Monsanto Company, 62 Maritime Drive, USA;(2) Monsanto Company, Chesterfield Campus, 700 Chesterfield Parkway North, MO, USA
Abstract:By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be carried by independent T-strands, which sometimes integrate in unlinked loci in the plant genome. This allows the recovery of marker-free transgenic plants through genetic segregation in the next generation. In this study, we have found that by repositioning the selectable marker gene in the backbone and leaving only the GOI in the T-DNA region, a regular two-border binary plasmid was able to generate marker-free transgenic maize plants more efficiently than a conventional single binary plasmid with multiple T-DNA borders. These results also provide evidence that both the right and left borders can initiate and terminate T-strands. Such non-canonical initiation and termination of T-strands may be the basis for the elevated frequencies of cotransformation and unlinked insertions.
Keywords:Agrobacterium-mediated transformation  Marker-free transformation  T-DNA border  T-DNA initiation  T-DNA linkage  T-DNA termination
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号