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A comparison between Andersen (ACFM) and Reuter Centrifugal Sampler (RCS-plus) for indoor sampling of airborne molds
Authors:G Wüst  H Friedl  D Haas  M Köck  F Pichler-Semmelrock  FF Reinthaler  R Schlacher  E Marth
Institution:(1) Insitute of Hygiene, Karl-Franzens-Universität Graz, Universitätsplatz 4, A-8010 Graz, Austria;(2) Institute of Statistics, Technical University Graz, Steyrergasse 17, A-8010 Graz;(3) Office of Hygiene of the Styrian Government, Austria
Abstract:This paper shows first results regarding thecollection efficiency and suitability of theAndersen-6-stage cascade impactor (ACFM,Graseby Andersen) and the Reuter CentrifugalSampler (RCS-plus, Biotest Diagnostics Corp.,Denville, NJ) in measuring concentrations ofindoor airborne viable mold spores. The ACFMshowed significantly higher numbers of colonyforming units than the RCS-plus. For exposureassessment the lower sampling efficiency of theRCS-plus in comparison to the ACFM is not soimportant, as no sampler is able to record theactual concentration of airbornemicroorganisms. For fungal exposure assessmentit is more important to compare indoor andoutdoor mold spore concentration and theindoors and outdoors occuring species. The ACFMdoes not overload as quickly as the RCS-plus,so there is not so much overgrowing andinhibition of colonies and it is easier tomaintain pure cultures. The advantages of theRCS plus are the simpler handling and, the optionof changing flow-rate. Even though each sampler hasits set of advantages and disadvantages, bothcan serve a useful function in airborne fungalspore sampling.
Keywords:Andersen impactor  cfu  indoor air  mold  RCS-plus  sampling efficiency
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