Molecular and immunological evaluation of the expression of cancer/testis gene products in human colorectal cancer |
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| Authors: | Pedro M. S. Alves Nicole Lévy Hanifa Bouzourene Sébastien Viatte Gabriel Bricard Maha Ayyoub Henri Vuilleumier Jean-Claude R. Givel Nermin Halkic Daniel E. Speiser Pedro Romero Frédéric Lévy |
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| Affiliation: | (1) NCCR, Molecular Oncology, ISREC, Ch. des Boveresses 155, 1066 Epalinges, Switzerland;(2) Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Ch. des Boveresses 155, 1066 Epalinges, Switzerland;(3) Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, Lausanne Branch, University Hospital (CHUV), 1005 Lausanne, Switzerland;(4) Department of Biochemistry, University of Lausanne, Ch. des Boveresses 155, 1066 Epalinges, Switzerland;(5) Institute of Pathology, University of Lausanne, rue du Bugnon 25, 1011 Lausanne, Switzerland;(6) Department of Visceral Surgery, University Hospital (CHUV), 1011 Lausanne, Switzerland |
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| Abstract: | Tumor-specific gene products, such as cancer/testis (CT) antigens, constitute promising targets for the development of T cell vaccines. Whereas CT antigens are frequently expressed in melanoma, their expression in colorectal cancers (CRC) remains poorly characterized. Here, we have studied the expression of the CT antigens MAGE-A3, MAGE-A4, MAGE-A10, NY-ESO-1 and SSX2 in CRC because of the presence of well-described HLA-A2-restricted epitopes in their sequences. Our analyses of 41 primary CRC and 14 metastatic liver lesions confirmed the low frequency of expression of these CT antigens. No increased expression frequencies were observed in metastatic tumors compared to primary tumors. Histological analyses of CRC samples revealed heterogeneous expression of individual CT antigens. Finally, evidence of a naturally acquired CT antigen-specific CD8+ T cell response could be demonstrated. These results show that the expression of CT antigens in a subset of CRC patients induces readily detectable T cell responses. |
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| Keywords: | Colorectal cancer Cancer-testis antigen T cell response |
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