Preparation of a high titre Salmonella abortusovis H-antigen and antiserum and improvement of the microagglutination test. |
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| Authors: | A Giannati G Koptopoulos E Hatzopoulou K Sarris |
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| Institution: | National Agricultural Research Foundation, Veterinary Institute of Infectious and Parasitic Diseases, Thessaloniki, Greece. |
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| Abstract: | Serological diagnosis of small ruminant abortions due to Salmonella abortusovis (S.a.o) was improved by using modified procedures to produce high titre H-antigens and anti-H serum. While the titres of the two standard antigens were 1:8 and 1:20, both modified antigens had a titre of 1:50. Similarly, the use of adjuvanted and non adjuvanted antigens for the production of anti-H hyperimmune serum resulted in a titre of 1:160,000 as compared to 1:20,000 when standard procedures were used. In addition, the slow micro-agglutination test has been modified and results can now be read within the same day. |
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