|
|||||
|
|
An investigation of a mouse model to estimate the potency of the diphtheria component in vaccines |
|
| Authors: | J G Kreeftenberg J van der Gun F R Marsman V M Sekhuis S K Bhandari S C Maheshwari |
| Affiliation: | 1. Department of Emergency Medicine, Aseer Central Hospital, Abha, Saudi Arabia;2. Department of Medicine, King Khalid University, Abha, Saudi Arabia;3. Department of Emergency Medicine, Armed forces hospital southern region, Khamis Mushait, Saudi Arabia;4. Emergency Medicine Department and Clinical Toxicology Division, King Abdulaziz Medical City, Ministry of National Guard-Health Affairs, Riyadh, Saudi Arabia |
| Abstract: | A mouse model to estimate the potency of the diphtheria toxoid components in vaccines using Vero cells to detect the neutralizing antibodies in the sera from immunized mice is described. The results obtained with this mouse model correlated significantly with those obtained in the lethal challenge test in guinea-pigs. For this reason it is suggested that the potency test in guinea-pigs be replaced by this mouse model because a considerable reduction in the number of animals used and costs can be achieved by the introduction of this mouse test for the routine control of the potency of the diphtheria component of vaccines. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |