Cytochemical demonstration of adenylate cyclase activity with cerium |
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| Authors: | L. Rechardt H. Hervonen |
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| Affiliation: | (1) Department of Biomedical Sciences, University of Tampere, P.O. Box 607, SF-33101 Tampere 10, Finland;(2) Department of Anatomy, University of Helsinki, Siltavuorenpenger 20, SF-00170 Helsinki 17, Finland |
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| Abstract: | Summary Cerium was applied for the ultrastructural, cytochemical localization of adenylate cyclase (EC 4.6.1.1.). The enzyme activity was stimulated with norepinephrine, prenalterol and choleratoxin in the brown fat cells of newborn rats. The final reaction product was observed in the plasmalemmas of the stimulated adipocytes. The precipitate was finely crystalline, easily visible in the electron microscope and in the X-ray microprobe analysis it yielded cerium and phosphate peaks, respectively. The use of cerium offers a new tool valid for the cytochemical localization of adenylate cyclase enzyme related to the membrane receptors.This study was supported by the grant from Reino Lahtikari Foundation |
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