| 葡萄糖对重组CHO细胞生长代谢及EPO表达的影响 |
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| 引用本文： | 孙祥明, 张元兴,.葡萄糖对重组CHO细胞生长代谢及EPO表达的影响[J].生物工程学报,2001,17(6):698-702. |
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| 作者姓名： | 孙祥明 张元兴 |
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| 作者单位： | 华东理工大学 |
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| 摘 要： | 在CHO细胞批培养中 ,葡萄糖浓度从8.9增加到49.6mmol/L ,最大活细胞密度没有明显的差异 ,乳酸对葡萄糖的得率系数首先随着葡萄糖浓度的增加而增加 ,葡萄糖浓度达到 17.9mmol/L后 ,乳酸对葡萄糖的得率系数基本上维持恒定。在本实验中 ,葡萄糖浓度对谷氨酰胺代谢没有明显的影响。EPO的累积浓度首先随着起始葡萄糖浓度的增加 ( 8.9～ 17.9mmol L)而增加 ,进而又随着葡萄糖浓度的增加 (17.9～ 49.6mmol L)而下降 ,表明存在一最适浓度 ,在此浓度下重组CHO细胞的EPO表达最大。
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| 关 键 词： | CHO细胞 葡萄糖 代谢 红细胞生成素 动物细胞培养 |
| 文章编号： | 1000-3061(2001)06-0698-05 |
| 修稿时间： | 2001年4月18日 |
Effects of Glucose on Growth, Metabolism and EPO Expression in Recombinant CHO Cell Cultures |
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| X M Sun,Y X Zhang.Effects of Glucose on Growth, Metabolism and EPO Expression in Recombinant CHO Cell Cultures[J].Chinese Journal of Biotechnology,2001,17(6):698-702. |
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| Authors: | X M Sun Y X Zhang |
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| Institution: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. |
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| Abstract: | The maximum viable cell density almost kept constant in the experimental range of initial glucose concentrations from 8.9 to 49.6mmol/L in the batch cultures of recombinant CHO cells. The yield coefficient of lactate to glucose increased with the initial glucose concentrations from 8.9 up to 17.9mmol/L, and then kept constant with above 17.9mmol/L. There was little effect of glucose on glutamine metabolism. It was also demonstrated that the accumulative concentration of EPO increased with the increase of glucose concentration from 8.9 to 17.9mmol/L, and decreased with its further increase from 17.9 to 49.6mmol/L. It is showed that there is an optimum glucose concentration at which the EPO express of cultured reombinant CHO cells is on the peak. |
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| Keywords: | CHO cell glucose metabolism erythropiotein animal cell culture |
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