葡萄糖对重组CHO细胞生长代谢及EPO表达的影响

在CHO细胞批培养中 ,葡萄糖浓度从8.9增加到49.6mmol/L ,最大活细胞密度没有明显的差异 ,乳酸对葡萄糖的得率系数首先随着葡萄糖浓度的增加而增加 ,葡萄糖浓度达到 17.9mmol/L后 ,乳酸对葡萄糖的得率系数基本上维持恒定。在本实验中 ,葡萄糖浓度对谷氨酰胺代谢没有明显的影响。EPO的累积浓度首先随着起始葡萄糖浓度的增加 ( 8.9～ 17.9mmol L)而增加 ,进而又随着葡萄糖浓度的增加 (17.9～ 49.6mmol L)而下降 ,表明存在一最适浓度 ,在此浓度下重组CHO细胞的EPO表达最大。

Effects of Glucose on Growth, Metabolism and EPO Expression in Recombinant CHO Cell Cultures

The maximum viable cell density almost kept constant in the experimental range of initial glucose concentrations from 8.9 to 49.6mmol/L in the batch cultures of recombinant CHO cells. The yield coefficient of lactate to glucose increased with the initial glucose concentrations from 8.9 up to 17.9mmol/L, and then kept constant with above 17.9mmol/L. There was little effect of glucose on glutamine metabolism. It was also demonstrated that the accumulative concentration of EPO increased with the increase of glucose concentration from 8.9 to 17.9mmol/L, and decreased with its further increase from 17.9 to 49.6mmol/L. It is showed that there is an optimum glucose concentration at which the EPO express of cultured reombinant CHO cells is on the peak.