Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides |
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| Authors: | Qiao-Ning Liu Rui-Sang Liu Yi-Hua Wang Zhi-Yuan Mi Dong-Sheng Li Jian-Jiang Zhong Ya-Jie Tang |
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| Institution: | 1. Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China;2. College of science, Jiangxi University of Agriculture, Nanchang 330045, China;3. Key Laboratory of Microbial Metabolism (Ministry of Education), College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;4. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | For the first time, a fed-batch fermentation process of Tuber melanosporum was developed for the efficient production of bioactive mycelia and Tuber polysaccharides. Each 1.67 g/L of peptone and 8.33 g/L of yeast extract were added on day 3, 6, and 9, respectively, and sucrose was fed to maintain its concentration around 35–5 g/L when its residual level decreased to 10–5 g/L. Then, the maximal biomass, the production of extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) reached 53.72 ± 2.57 g DW/L, 7.09 ± 0.62 and 4.43 ± 0.21 g/L, respectively. Compared with the batch culture conducted in the enriched medium, the biomass, the production of EPS and IPS were enhanced by 55.8%, 222.3% and 103.2%, respectively. Not only the cell density but also the production of EPS and IPS were the highest ever reported in truffle fermentation, and the biomass was also the highest as ever reported in mushroom fermentation. |
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| Keywords: | Medicinal mushroom Tuber melanosporum Fed-batch fermentation Tuber polysaccharides |
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