Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: Purification,characterization and potential application as a laundry detergent additive |
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| Authors: | Anissa Haddar Rym AgrebiAli Bougatef Noomen HmidetAlya Sellami-Kamoun Moncef Nasri |
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| Affiliation: | Laboratoire de Génie Enzymatique et de Microbiologie -Ecole Nationale d’Ingénieurs de Sfax, B.P. “W” Sfax 3038, Tunisia |
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| Abstract: | Two detergent stable alkaline serine-proteases (BM1 and BM2) from Bacillus mojavensis A21 were purified. The molecular weights of BM1 and BM2 enzymes determined by SDS–PAGE were approximately 29,000 Da and 15,500 Da, respectively. The optimum pH values of BM1 and BM2 proteases were shown to be 8.0–10.0 and 10.0, respectively. Both enzymes exhibited maximal activity at 60 °C, using casein as a substrate. |
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| Keywords: | Alkaline proteases Surfactant-stable Detergent activity Bacillus mojavensis |
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