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Characterization and fouling properties of exopolysaccharide produced by Klebsiella oxytoca
Authors:Lei Feng  Xiufen Li  Guocheng Du  Jian Chen
Affiliation:1. Lab of Environmental Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China;2. Resource and Environment College, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, PR China;3. School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China;4. National Key Lab of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China
Abstract:Klebsiella oxytoca produced a type of exopolysaccharide (EPS) with the average molecular weight (Mw) of 116,018 Da and the average size of 260 nm. The EPS monosaccharide components contained rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose and the molar ratio among them was 0.033:0.0411:0.0147:0.0051:0.2393:0.0986:0.1304. Typical EPS absorption peaks in FT-IR spectrum and pseudoplastic properties were also revealed. The polyvinylidenefluoride (PVDF) membrane showed a relatively larger flux decline resulted from the EPS fouling. The EPS filtration was dominated by more than one mechanism at the beginning phase and mainly by the cake formation at the later phase for both membranes. The pore blocking resistance had a predominant contribution to the filtration resistance and the cake resistance played a secondary role for both the membranes. The EPS adsorption resulted in a weak membrane fouling. The PVDF membrane exhibited a larger adsorption resistance than the polypropylene (PP) membrane.
Keywords:Klebsiella oxytoca   Exopolysaccharide   Biofouling   Microfiltration   Resistances
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