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Screening of Saccharomyces cerevisiae strains with respect to anaerobic growth in non-detoxified lignocellulose hydrolysate
Authors:João RM Almeida  Kaisa KarhumaaMarie-F Gorwa-Grauslund
Institution:Department of Applied Microbiology, Lund University, P.O. Box 124, S-221 00 Lund, Sweden
Abstract:A microplate screening method was used to assess anaerobic growth of 12 Saccharomyces cerevisiae strains in barley straw, spruce and wheat straw hydrolysate. The assay demonstrated significant differences in inhibitor tolerance among the strains. In addition, growth inhibition by the three hydrolysates differed so that wheat hydrolysate supported growth up to 70%, while barley hydrolysate only supported growth up to 50%, with dilute-acid spruce hydrolysate taking an intermediate position.
Keywords:Saccharomyces cerevisiae  Yeast  Screening  Lignocellulose hydrolysate  Bioethanol
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