The bioleaching feasibility for Pb/Zn smelting slag and community characteristics of indigenous moderate-thermophilic bacteria |
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| Authors: | Yi Cheng Zhaohui Guo Xueduan Liu Huaqun Yin Guanzhou Qiu Fengkai Pan Hongwei Liu |
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| Institution: | 1. Institute of Environmental Engineering, School of Metallurgical Science and Engineering, Central South University, Changsha 410083, PR China;2. Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University, Changsha 410083, PR China |
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| Abstract: | The feasibility of recovering metal values and removing hazardous elements from the Pb/Zn smelting slag using bioleaching technique were studied through a flask experiment, and the community characteristics of the indigenous moderate-thermophilic bacteria in this bioleaching system were also analyzed through a culture-independent restriction fragment length polymorphism (RFLP) of 16S rRNA genes approach. The results show that more than 80% of Al, As, Cu, Mn, Fe and Zn in the Pb/Zn smelting slag were leached at 65 oC, pH 1.5, pulp density 5%, but only about 5% of Pb. Phylogenetic analysis revealed that the bacteria in the bioleaching system mainly fell among Firmicutes, Gammaproteobacteria and Betaproteobacteria, and the dominant bacteria are affiliated with Bacillus spp., Sporosarcina spp. and Pseudomonas spp. |
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| Keywords: | Moderate-thermophilic bacteria Bioleaching Pb/Zn smelting slag 16S rRNA-RFLP Phylogenetic analysis |
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