Modification of wheat straw lignin by solid state fermentation with white-rot fungi |
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Authors: | Maria J. Dinis,Rui M.F. Bezerra,Fernando Nunes,Albino A. Dias,Cristina V. Guedes,Luí s M.M. Ferreira,John W. Cone,Guilhermina S.M. Marques,Ana R.N. Barros,Miguel A.M. Rodrigues |
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Affiliation: | 1. CECAV – Universidade de Trás-os-Montes e Alto Douro, Department of Animal Science, Apartado 1013, 5001-801 Vila Real, Portugal;2. CITAB – Universidade de Trás-os-Montes e Alto Douro, Department of Biological and Environmental Engineering, Apartado 1013, 5001-801 Vila Real, Portugal;3. CQ – Universidade de Trás-os-Montes e Alto Douro, Department of Chemistry, Apartado 1013, 5001-801 Vila Real, Portugal;4. Animal Nutrition Group, Department of Animal Sciences, Wageningen University, The Netherlands;5. Universidade de Trás-os-Montes e Alto Douro, Department of Agronomy, Apartado 1013, 5001-801 Vila Real, Portugal |
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Abstract: | The potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (Trametes versicolor, Bjerkandera adusta, Ganoderma applanatum and Phlebia rufa), was exploited to modify wheat straw cell wall. At different fermentation times, manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), laccase, carboxymethylcellulase (CMCase), avicelase, xylanase and feruloyl esterase activities were screened and the content of lignin as well as hydroxycinnamic acids in fermented straw were determined. All fungi secreted feruloyl esterase while LiP was only detected in crude extracts from B. adusta. Since no significant differences (P > 0.05) were observed in remaining lignin content of fermented straw, LiP activity was not a limiting factor of enzymatic lignin removal process. The levels of esterified hydroxycinnamic acids degradation were considerably higher than previous reports with lignocellulosic biomass. The data show that P. rufa, may be considered for more specific studies as higher ferulic and p-coumaric acids degradation was observed for earlier incubation times. |
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Keywords: | Wheat straw White-rot fungi Biodegradation |
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